The comet assay: a heavenly method!
نویسنده
چکیده
The contributions to this special issue of Mutagenesis have been selected to cover the main research areas served by the comet assay, namely genotoxicology, environmental toxicology, human biomonitoring and fundamental investigations into mechanisms of DNA damage and repair. Innovative methods are described, technical issues are explored, and guidelines are given for venturing into relatively new or unexploited areas of research. The popularity of the comet assay in a historical context is illustrated by a bibliometric survey.
منابع مشابه
Comet assay as a sensitive technique in occupational health studies; A literature review
Background: Occupational health is a sensitive field in many countries where occupational exposure needs attention due to the lack of awareness. Several businesses, including leather, textile, iron, steel, and dyeing industries use a variety of mutagenic and genotoxic chemicals. Exposure to these chemicals may pose serious health risks, including genetic damage to workers. Comet assay is a tech...
متن کاملStudying of the Expression of BAX and BCL-2 Genes in Men with Sperm DNA Fragmentation Based on DFI and Comet Assay
Introduction: The amount of expression of BAX and BCL-2 genes in infertile men’s sperm as well as its association with sperm parameters and DNA fragmentation index is an issue which has not been studied yet. In this research, it is assumed that up-regulation of BAX and downregulation of BCL-2 are directly associated with sperm DNA fragmentation. Methods: After obtaining semen samples from the ...
متن کاملA Study of Cytogenetic Stability of Induced Pluripotent Stem Cells Using Karyotyping and Comet Assay Techniques
Background & Aims: Induced pluripotent stem cells (iPSCs) have the capability to undergo unlimited selfrenewal and differentiation into all cell types in the body. These cells are artificially derived from a nonpluripotent cell, typically human dermal fibroblasts (HDFs). The study of cytogenetic stability of these cells, in order to use iPS cells and apply studies in therapeutic applications, i...
متن کاملCytogenetic damage induced by crude oil in Anodonta cygnea (mollusca,bivalvia) assessed by the comet assay and micronucleus test
Crude oil is enriched in polycyclic aromatic hydrocarbons (PAHs). Many PAH analogs have proved to potentially damage DNA. DNA damage can be assessed using various biomarkers to find out the degree of genotoxicity of pollutants following in vitro exposure. In this research the comet assay and micronucleus (MN) test were used to detect DNA damages and cytogenetic changes following crude oil expos...
متن کاملProtective effects of phenolic acids on mercury-induced DNA damage in precision-cut kidney slices
Objective(s): Precision-cut tissue slices are considered an organotypic 3D model widely used in biomedical research. The comet assay is an important screening test for early genotoxicity risk assessment that is mainly applied on in vitro models. The aim of the present study was to provide a 3D organ system for determination of genotoxicity using a modified method of the comet assay since the s...
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ورودعنوان ژورنال:
- Mutagenesis
دوره 30 1 شماره
صفحات -
تاریخ انتشار 2015